Emerging Influenza Reagents When You Need Them

Ready-to-use Reagents for H5N1 Influenza Research

Research on emerging influenza strains presents challenges that may put this important work out of your reach. Attenuated influenza strains can be difficult to access and time-consuming to bank. BSL-3 facilities are unavailable to many.

But did you know there’s an easier way to get started with microneutralization and HAI assays? With our non-replicative, BSL-2 safe reagents, your team can be running H5N1 avian influenza assays in as little as 10 days.

Schematics of Influenza TiterSafe and RVP. Both particles have HA and NA proteins in a lipid bilayer surrounding a lentivirus core. RVPs contain a luciferase or GFP reporter gene; TiterSafe particles lack genetic information.

For your influenza research needs, we offer two easy-to-use reagents for fast results: Reporter Virus Particles (RVPs) for viral neutralization and infectivity assays, and TiterSafe™ for HAI assays. Both are available strain-matched to a variety of H5N1 and other emerging strains, and they’re ready to ship.

Influenza TiterSafe Particles for HAI Assays

Influenza TiterSafe is a cost-effective reagent that safely replaces live influenza virus in HAI assays. It arrives ready to use, with viral titers that are equivalent to commercially available inactivated influenza virus.
Interactions and the corresponding microtiter well results for three conditions in an HAI assay. RBC-only negative control, agglutination with a matrix of RBCs and TiterSafe particles, and agglutination inhibition with serum antibodies blocking interactions between TiterSafe particles and RBCs.

Influenza TiterSafe Particles produce easy-to-read results in your existing HAI workflow

Influenza Reporter Virus Particles for Microneutralization Assays

Non-replicative Reporter Virus Particles (RVPs) are a safe, easy-to-use substitute for live virus in neutralization assays. When an RVP infects a cell, the reporter integrates into the cell’s genome. The cell then expresses the gene, producing an optical readout. Easy to read on standard equipment such as a microscope, flow cytometer, or luminescence plate reader, the optical reporter eliminates the need for staining or counting viral plaques.
Neutralization assay setup in a 96-well plate. Step 1: add 2x dilutions of antibody or serum. Step 2: add pseudovirus and incubate. Step 3: Add equivalent of target cells. Step 4: Incubate 24 to 48 hours. Step 5: Analyze for reporter expression.

Influenza pseudovirus neutralization assay at a glance. RVPs are available with a GFP or luciferase reporter. Reporter expression indicates when cells have been infected successfully. GFP read-out can be assessed by microscopy or flow cytometry, and luciferase readout can be assessed by plate reader.

Emerging Influenza Strain Product Catalog

Influenza A Subtype Strain Information RVP Catalog No. Titersafe Catalog No.
H5N1 Texas/37/2024 RVP-1218 HAP-1218
H5N1 dairy_cattle/Texas/24-008749-003-original/2024 RVP-1217 HAP-1217
H5N1 American Wigeon/SC/22-000345-001/2021 RVP-1214 HAP-1214
H5N8 Astrakhan/3212/2020 RVP-1209 –
H7N9 Guangdong/17SF003/2016 RVP-1210  –
H7N9 Suzhou/3/2013 RVP-1204 –
H5N1 turkey/Turkey/1/2005 RVP-1208 –
H5N1 Indonesia/5/05 RVP-1201 –
H5N1 Vietnam/1194/2004 RVP-1207* –
H5N1 Vietnam/1203/2004 RVP-1205 HAP-1205

*Available in GFP only
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All of our virology catalog offerings are produced using ISO 9001-certified processes and QMS. Because we know your time is valuable, our experts are here to help make sure your experiments work the first time.

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