In Stock

SARS-CoV-2 D614G Reporter Virus Particles

SKU:
RVP-702
Maximum Purchase:
10 units
Current Stock:
Virus:
SARS-CoV-2
Strain:
D614G
Unit:
1 mL
Lead Time:
Ready to ship
$616.00
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Neutralization utilized 5 µl of SARS-CoV-2 RVPs in a 384-well plate. GFP positive cells were detected with an Intellicyt iQue flow cytometer using the BL-1 channel (Ex. 488 nm, Em. 530).
Infectivity and neutralization determined in HEK-293T cells stably over-expressing ACE2 (Integral Cat# C-HA102). Infectivity data represents the average of three independent vials, each tested in quadruplicate.
Reporter Virus Particles (RVP) by Integral Molecular, Inc.

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Neutralization utilized 5 µl of SARS-CoV-2 RVPs in a 384-well plate. GFP positive cells were detected with an Intellicyt iQue flow cytometer using the BL-1 channel (Ex. 488 nm, Em. 530).
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Integral Molecular’s SARS-CoV-2 Reporter Virus Particles (RVPs) are replication-incompetent pseudotyped virus particles that enable safe (BSL-2), easy, and high-throughput viral infectivity and neutralization assays using standard detection instrumentation. SARS-CoV-2 RVPs display antigenically correct spike protein on a heterologous virus core and carry a modified genome that expresses a convenient optical reporter gene within 24 hours of cellular infection. Protocols are provided for neutralization and infectivity assays. 

Target cells are sold separately. 

Product SARS-CoV-2 Reporter Virus Particles (RVPs)
Catalog Number RVP-702
Strain D614G
Packaging 20% FBS/DMEM
Recommended Input Range 10-15 uL per well (96-well plate) using the 293T-ACE2 stable cell line
Target Cell Line 293T-hsACE2 Clonal Cell Line (Cat# C-HA102)

Mutations

(compare to GenBank QHD43416.1)

 D614G
Lead time Ready to ship
Shipping Shipped on dry ice
Storage conditions Store at -80°C (+/- 10°C) upon receipt
Size 1.0 mL/vial

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