Ready Reporter Virus™ Kit SARS-CoV-2 D614G

Integral Molecular’s Ready Reporter Virus™ kit for SARS-CoV-2 D614G is an all-in-one, thaw-and-use solution for fast neutralization results in <1 day. Each single-use kit comes with non-replicative Ready Reporter Virus (RRV) particles, permissive target cells, and fusion signal enhancer in sufficient quantity for a 96-well plate. Validated control antibodies are available to purchase separately (see below). 

The thaw-and-use target cells are pre-loaded with a reporter dye that remains uncleaved until RRV particles deliver a reporter enzyme to the cell. Upon infection, the enzyme cleaves the dye, causing a shift in its emission wavelength.   

• Recommended dye excitation is 409 nm 
• Emission wavelength of infected cells with cleaved dye is 447 nm 
• Emission wavelength of uncleaved dye is 520 nm 

We offer academic discounts up to 20% to support your research.  

Interested in testing how Ready Reporter Virus™ kits perform in your lab?  Request a sample kit today! 

Kit Components 

RRV kit components are for single use. Re-freezing risks complete loss of viral activity and cell viability. 

Validated control antibodies. Must be purchased separately. 

*Negative control for virus-specific neutralization 

SARS-CoV-2 D614G Ready Reporter Virus™ (RRV) Particles are non-replicative BSL-2 safe pseudoviral particles that enable an enzyme-catalyzed optical readout compatible with flow cytometry, microscopy, or a plate reader. RRV particles display antigenically equivalent viral protein(s) on a heterologous virus core and produce a fluorescent readout within 3 hours of cellular infection.  

Dye-loaded HEK-ACE2 Target Cells are a monoclonal cell line of human embryonic kidney cells (HEK-293T) that overexpress human angiotensin-converting enzyme 2 (hACE2, GenBank Accession# NM_021804.3) to facilitate the binding and entry of SARS-CoV-2. The cells are loaded with a tandem dye substrate. Cells should be stored at -80°C for storage up to 3 months. For longer term storage, LN2 may extend kit life.  Cells are intended for research purposes only.    

100X Fusion Signal Enhancer allows the enzymatic reaction signal to be read easily by common fluorescence detection methods. 

Protocols are provided for neutralization and infectivity assays. Please refer to the protocols linked below for additional assay details including recommended buffer and plasticware.