Integral Molecular’s Animal Coronavirus Reporter Virus Particles (RVPs) are replication-incompetent pseudotyped virus particles that enable safe (BSL-2), easy, and high-throughput viral infectivity and neutralization assays using standard detection instrumentation. RVPs for animal coronaviruses are a valuable tool to assess breadth of neutralization for therapeutics against human coronaviruses or to identify antibodies that target conserved regions in coronaviruses. Animal CoV RVPs display antigenically correct spike protein on a heterologous virus core and carry a modified genome that expresses a convenient optical reporter gene within 24 hours of cellular infection.
Protocols are provided for neutralization and infectivity assays.
| Product | Pangolin Coronavirus, PCoV_GX/P5L Reporter Virus Particles (RVPs) |
| Catalog Number | RVP-812 |
| Isolate |
PCoV_GX/P5L
|
| Species of Isolation |
Manis javanica (Malayan Pangolin)
|
| Packaging | 20% FBS/DMEM |
| Recommended Input Range | 10 uL per well (96-well plate) using the 293T-ACE2 stable cell line |
| Target Cell Line | 293T-hsACE2 Clonal Cell Line (Cat# C-HA102) |
| Lead time | GFP Ready to ship, luciferase 1-4 weeks |
| Shipping | Shipped on dry ice |
| Storage conditions | Store at -80°C (+/- 10°C) upon receipt |
| Size | 1.0 mL/vial |
| Warranty | Terms and Conditions (pdf) |
| SDS | Animal CoV Safety Data Sheet (pdf) |
| Protocols | |
| Technical Data Sheets | Animal CoV RVP Tech Data Sheets |
