In Stock

Neutralization Kit - SARS-CoV-2 D614G

SKU:
KIT-702
Maximum Purchase:
10 units
Current Stock:
Virus:
SARS-CoV-2
Kit RVP Strain:
D614G
RVP Unit:
1 mL
Lead Time:
Ready to ship
First Purchase Discount Code:
FIRSTKIT50 for 50% off
$1,530.00
Request a quote, including discounts on bulk orders
D614G variant SARS-CoV-2 Neutralization Kit by Integral Molecular. Tubes of cells, antibody and pseudovirus are displayed in a box. Use code FIRSTKIT50 for 50% off your first purchase.
Neutralization utilized 5 µl of SARS-CoV-2 RVPs in a 384-well plate. GFP positive cells were detected with an Intellicyt iQue flow cytometer using the BL-1 channel (Ex. 488 nm, Em. 530).
Neutralization experiments were conducted using 5µL of SARS-CoV-2 RVPs (Omicron BA.1, INTG Cat # RVP 768G; Omicron BA.2, INTG Cat # RVP 770G; Omicron BA.2.75, INTG Cat # RVP 776G; Delta, INTG Cat # RVP 763G; D614G, INTG Cat # RVP 702G) in a 384-well plate using HEK-293T monoclonal cells stably overexpressing ACE2 (INTG Cat # CHA102).
Infectivity is determined in HEK-293T cells. Data are based on three independent tubes, each tested in quadruplicate.
Flow cytometry analysis of 293T-hsACE2 cells (Cat# C-HA102) with an ACE2 specific monoclonal antibody (R&D Systems Cat# MAB9332-100). Detection using a secondary antibody labelled with AlexaFluor 488 (Jackson ImmunoResearch Cat# 115-545-003). Fluorescence was detected with an Intellicyt iQue flow cytometer using the BL-1 channel (Ex. 488 nm, Em. 530).

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D614G variant SARS-CoV-2 Neutralization Kit by Integral Molecular. Tubes of cells, antibody and pseudovirus are displayed in a box. Use code FIRSTKIT50 for 50% off your first purchase.
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Integral Molecular’s D614G Neutralization Kit is an all-in-one solution for easy neutralization assays using replication-incompetent pseudoviruses. The kit includes SARS-CoV-2 D614G Reporter Virus Particles, a clonal cell line overexpressing the SARS-CoV-2 receptor, a SARS-CoV-2 neutralizing antibody against early variants, and Reporter Virus Particles expressing VSV-G as a negative control.

Catalog Number Product Unit
RVP-702G/L SARS-CoV-2 D614G Reporter Virus Particles  2 vials (1 mL each)
C-HA102 293T-hsACE2 Clonal Cell Line  1 vial (3e6 cells) 
IM-COV2-G06 SARS-CoV-2 Broadly Neutralizing Antibody (including Delta)  1 vial (100 ug) 
RVP-1002G/L  VSV Reporter Virus Particles with MLV core  1 vial (1 mL)

Reporter Virus Particles (RVPs) are replication-incompetent pseudotyped virus particles that enable safe (BSL-2), easy, and high-throughput viral infectivity and neutralization assays using standard detection instrumentation. RVPs display antigenically equivalent membrane protein(s) on a heterologous virus core and carry a modified genome that expresses a convenient optical reporter gene within 24 hours of cellular infection.  

SARS-CoV-2 RVPs display antigenically correct spike protein on a heterologous virus core ideal for SARS-CoV-2 neutralization assays.  

C-HA102 is a monoclonal cell line of human embryonic kidney cells (HEK-293T) that overexpress human angiotensin-converting enzyme 2 (hACE2, GenBank Accession# NM_021804.3) to facilitate the binding of SARS-CoV-2 Reporter Virus Particles. This clone stably expresses hACE2 for up to 30 passages under puromycin selection and was selected for its transduction efficiency by SARS-CoV-2 RVPs. The cells are adherent and have an epithelial morphology.  

IM-CoV2-G06 is a recombinant monoclonal antibody that neutralizes early SARS-CoV-2 variants including Wuhan-Hu-1, D614G, Alpha, and Delta. This clone weakly neutralizes Omicron variants BA.1 and BA.1.1. 

VSV RVPs function as a control for RVP experiments to differentiate specific versus non-specific effects on neutralization. VSV RVPs display VSV-G on a heterologous MLV core. 

Cells are intended for research purposes only. Protocols are provided for neutralization and infectivity assays. 

Product Neutralization Kit - SARS-CoV-2 D614G
Catalog Number KIT-702
Strain D614G
Packaging 20% FBS/DMEM
Recommended Input Range 10-15 uL per well (96-well plate) using the 293T-ACE2 stable cell line
Lead time Ready to ship
Shipping Shipped on dry ice
Storage conditions Store at -80°C (+/- 10°C) upon receipt
Size 5 total vials

Integral Molecular’s products for SARS-CoV-2 neutralization assays have been cited in leading publications describing both vaccine and therapeutic antibody development. Example publications include:

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