In Stock

293T-hsACE2 Clonal Cell Line

SKU:
C-HA102
Maximum Purchase:
5 units
Current Stock:
For use with:
SARS-CoV-2 RVPs
Unit:
2 vials x 3e6 cells each
Lead Time:
Ready to ship
$1,725.00
Flow cytometry analysis of 293T-hsACE2 cells (Cat# C-HA102) with an ACE2 specific monoclonal antibody (R&D Systems Cat# MAB9332-100). Detection using a secondary antibody labelled with AlexaFluor 488 (Jackson ImmunoResearch Cat# 115-545-003). Fluorescence was detected with an Intellicyt iQue flow cytometer using the BL-1 channel (Ex. 488 nm, Em. 530).
Vials of cells by Integral Molecular, Inc.

Frequently Bought Together:

Flow cytometry analysis of 293T-hsACE2 cells (Cat# C-HA102) with an ACE2 specific monoclonal antibody (R&D Systems Cat# MAB9332-100). Detection using a secondary antibody labelled with AlexaFluor 488 (Jackson ImmunoResearch Cat# 115-545-003). Fluorescence was detected with an Intellicyt iQue flow cytometer using the BL-1 channel (Ex. 488 nm, Em. 530).
Inc. Tax
Ex. Tax

For your best signal in SARS-CoV-2 neutralization or infectivity assays, use our 293T-hsACE2 monoclonal cell line. This HEK-293T human embryonic kidney cells overexpresses human angiotensin-converting enzyme 2 (hACE2, GenBank Accession# NM_021804.3) to facilitate the binding of SARS-CoV-2 Reporter Virus Particles. 

This clone stably expresses ACE2 for up to 30 passages under puromycin selection and was selected for its transduction efficiency by SARS-CoV-2 RVPs. The cells are adherent and have an epithelial morphology. 

Cells are intended for research purposes only. 

For more details about RVPs, their applications, and answers to frequently asked questions, visit the Reporter Virus Particle Information page. For tips on setting up your own neutralization assay, visit Pseudovirus Neutralization Assays: A How-to Guide for Getting Started. 

Product 293T-hsACE2 clonal cell line
Catalog Number C-HA102
Parental Cell Line HEK 293T
Transgene hsACE2 (NM_021804.3)
Media Cell Freezing Media (70% Cell Culture Media, 20% FBS, 10% DMSO)
Lead time Ready to ship
Shipping Shipped on dry ice
Storage conditions Liquid nitrogen vapor phase
Size 1.0 mL/vial (3e6 cells), 2 vials
Biosafety Level BSL-2